Transition from Cellulose I Family to Cellulose II Family
نویسندگان
چکیده
منابع مشابه
Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.
A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus Trichoderma reesei. Expression of the hybrid gene in Escherichia coli yielded up to 50 mg of the hybrid protein, CexCBDCbhI, per liter of culture supernatant. The hybrid was purified to homogeneity by a...
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ژورنال
عنوان ژورنال: Polymer Journal
سال: 1987
ISSN: 0032-3896,1349-0540
DOI: 10.1295/polymj.19.855